Animal Cap Explant Induction Using Anion Exchange Resin Beads
4:58
Animal Cap Dissociation and Reaggregation
6:52
Animal Cap Explant Implantation onto Xenopus laevis Embryo Neural Plates
8:12
Results: Representative Xenopus Explants
10:13
Conclusion
Transkript
The overall goal of this protocol is to use x laevis blastocoel roof or animal cap explants for testing the molecular mechanisms and cellular processes underlying neural development in vitro and in vivo. These methods can help answer key questions
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In Xenopus embryos, cells from the roof of the blastocoel are pluripotent and can be programmed to generate various tissues. Here, we describe protocols to use amphibian blastocoel roof explants as an assay system to investigate key in vivo and in vitro features of early neural development.