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JoVE Journal

Neuroscience

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3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

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10:14 min

May 12th, 2019

May 12th, 2019

7,145 Views

0:04

Title

1:09

Brain Surface Scan, Slicing, and Multi-electrode Array Recording

5:23

MRI Image Processing to Stripe Cortical Surfaces

6:32

Preprocessing for 3D Scan Data and Coregistration of the MRI Surface and the 3D Scan Surface

8:18

Results: Overlapping Accuracy Between MRIs and 3D Scans

9:17

Conclusion

Transkript

This protocol is the first to introduce 3D scanning technology in the anatomical, specifically neuroanatomical research field, and has achieved highly accurate overlapping performance. The 3D neuro embedding overlap, or 3D neuro protocol, embeds m

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This article introduces an experimental protocol using 3D scanning technology bridging two spatial scales: the macroscopic spatial scale of whole-brain anatomy imaged by MRI at >100 μm and the microscopic spatial scale of neuronal distributions using immunohistochemistry staining and a multielectrode array system and other methods (~10 μm).

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