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Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation

7.0K Views

09:50 min

October 27th, 2019

October 27th, 2019

6,956 Views

0:04

Title

1:37

Amoeba Culture

2:44

Propagation of Stock Virus in Amoebae

3:33

End-point Dilution and Single Cell Micro-aspiration

6:09

PCR Screening

7:06

Results: Optimization of the Isolation of Novel Viruses

8:17

Conclusion

Transkript

Single Cell Micro-Aspiration is a method to complement the different techniques used in the field of microbiology in general and in virology in particular. It could answer key question and resolve problems encountered during giant viruses isolatio

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Here, we describe a single cell micro-aspiration method for the separation of infected amoebae. In order to separate viral subpopulations in Vermamoeba vermiformis infected by Faustoviruses and unknown giant viruses, we developed the protocol detailed below and demonstrated its ability to separate two low-abundance novel giant viruses.

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