A New Optokinetic Testing Method to Measure Rat Vision

Summary

The Optokinetic Nystagmus (OKN) behavioral testing method is used for the assessment of visual acuity in rodents. Here a simple method is demonstrated that can be easily set up in research laboratories for a reliable assessment of visual function in both normal and experimental rats.

Abstract

Optokinetic nystagmus (OKN) is a reflexive eye movement initiated by the motion of visual stimuli in the field of vision. The head-tracking movement associated with OKN is commonly used as a measure of visual function in rodents. To record OKN responses in normal and experimental rats, a simple and inexpensive apparatus has been developed. This setup uses two tablet screens to display the OKN visual stimulus consisting of high contrast black and white stripes generated using the OKN Stripes Visualization Web Application, a freely available software. The rat is placed inside a clear Plexiglass holder that limits movement so that the rat's head continuously faces the OKN display screen. The position of the rat holder can be changed to adjust the distance between the rat and the display screen. A micro-camera positioned above the rat holder is used to record the rat's visual activities. These recordings can be used for quantitative assessments. Based on the presence or absence of clear head-tracking, the OKN responses at different spatial frequencies can be determined. The collected data demonstrates a novel technique for reliable measurement of visual acuity in normal and retinal degenerate rats.

Introduction

When the eye is exposed to sustained full-field visual motion, a distinct pattern of fast and smooth tracking eye movements and low-acceleration head movements emerge in the direction of visual motion, called optokinetic nystagmus (OKN)^1,2. The neurological pathway of OKN passes from the retina to the lateral geniculate body, occipital lobe, and cerebellar flocculus and connects to the ocular motor neurons³. Neural damage anywhere along these neural pathways may lead to changes in the OKN responses. The OKN response is used as a tool to assess cerebral symmetry, psychogenic blindness, and visual acuity in human patients^4,5. Visual acuity is assessed by quantifying functional responses, which can be integral in determining the success of treatments and experiments centered around restoring vision lost due to neurodegenerative diseases^3,6,7. In animals, OKN responses can be used to accurately assess visual acuity, which provides researchers with the ability to collect both quantitative and qualitative data regarding visual function. In rodents, it is possible to measure the visual acuity of the left and right eyes independently based on the direction of rotation of the stripes both in the clockwise and counter-clockwise directions⁸. This counter-clockwise and clockwise motion exposes each eye to either naso-temporal (N-T) or tempo-nasal (T-N) motion⁹, respectively. The T-N stimulus results in significantly higher response compared to the N-T stimulus since rodents are more sensitive to the dangers coming from behind or from the side.

Previously, visual function in normal laboratory rats and retinal degenerate rats was tested using different OKN testing methods^{6,10,11,12,13}. However, certain variabilities in the visual acuity scores are observed between different studies, including the data which is shown in the present investigation. This variability can be mostly attributed to the differences in the testing setup used. Differences in the size of the testing arena and the type of the OKN visual stimuli used^6,10 can be the major factors. The stimuli used in these experiments include sinewave gratings for the appearance of a virtual cylinder¹⁴, interchangeable rotating cylinders¹⁵, and high contrast (black and white) stripes displayed on four computer monitors¹⁰. Major limitations associated with these OKN testing apparatus and methods include the large size of the equipment, movement of the animals in the testing arena, and frequent incidence of the animal falling from the testing platform^7,11,12.

To minimize the above limitations, a new apparatus for OKN testing in rats has been developed. This apparatus is comparatively inexpensive, proven to be efficient, easy to operate, and enables assessment of visual function (Figure 1). The apparatus uses two tablet screens to display the OKN visual stimuli (visualization software) at different spatial frequencies. A micro-camera is used to record the animal's activities during testing for later analysis of the data. With the objective of making an easy-to-set up OKN apparatus in research laboratories, this new setup outlines critical modifications to the existing OKN testing apparatus. The OKN stimuli used here consists of black and white stripes at different spatial frequencies and different directions of rotation (left to right or right to left). The major component of the OKN testing apparatus includes two touchscreen tablet screens (7.9 inches) used to display the OKN stimuli (Figure 2). Two adjustable holders are used to hold the tablet screens in the desired position. The holders are securely attached to the edge of a procedure table that allows adjustment of its height and angle. The rats are placed in a rat holder that faces the display screens. The rat holders are made of transparent plastic (polymethyl methacrylate) tube. The holder is attached to a pedestal and a metal stand to ensure stable placement on the procedure table. The size of the holding tubes varies from 4 to 6 inches in length and 2.5 to 3 inches in diameter, depending on the size of the rats used. The distance between the rat and the display screen is adjusted by changing the position of the rat holder. The rat holder helps to maintain continuous exposure of the rat's head toward the display screens and reduces its movements during testing. A micro-camera is used to record the head tracking responses. The shortcomings of this new setup include different refresh rates for the screen and the possibility of optical illusions when using narrow stripes. However, these can be considered as common issues associated with a computer-based OKN setup. In addition to the above issues, in the current setup, rats are not tested using a virtual cylinder¹⁴ that influences the optimum OKN response. The novelty of this method lies within the technique and apparatus in which the method is employed. This technique can be easily set up in research laboratories for reliable visual acuity measurement in rodents.

Protocol

All animal procedures were performed in compliance with the experimental guidelines approved by the regional authorities and accepted by the Institutional Animal Care and Use Committee (IACUC) at the University of Southern California (USC), and conformed to the Association for Research in Vision and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Vision Research and the European directive 2010/63/EU on the protection of animals used for scientific purposes.

NOTE: The rats used in this study are pigmented retinal degenerate Royal College of Surgeons (RCS) rats and Long Evans (LE) rats. Figure 3 shows a schematic illustration showing different stages of OKN testing and analysis.

1. Procedures

1. Preparation of the setup
   1. Keep the rat in the cage and for 30 min inside the testing room with the room lights turned off.
      NOTE: This helps to minimize the stress that occurs during transportation and provides a uniform lighting condition for all experiments.
   2. Set the position of the tablet screens facing each other at 155° to display the OKN Stripes Visualization Web Application.
   3. Place the rat holder in the middle of two tablet screens at 4.5 inches from the center.
      NOTE: At this position, the rat's head will be about 3.5 inches away from the display screen.
   4. Click on OKN Stripes Visualization Web Application from the desktop of the tablet to display visual stimuli (black and white stripes) starting with the lowest spatial frequency.
      NOTE: Spatial frequencies 0.08, 0.15, 0.2, 0.24, 0.28, 0.33, 0.38 cycles per degrees (c/d) are used, starting at the lowest spatial frequency (0.08c/d) and changing to higher spatial frequencies (up to 0.38 c/d). At each spatial frequency, rats are tested for both clockwise and counter-clockwise directions in rotation of stripes (1 min each).
2. OKN testing procedure
   1. Select a rat holder of a suitable size.
   2. Bring the rat toward the opening of the rat holder and carefully guide the rat to the inside.
   3. Allow the rat to settle inside the holder (1-2 min) prior to testing.
      NOTE: By proper handling, it is possible to maintain the rat inside the holder in most of the cases; when necessary, the rat holder is held high (up to 2 feet above the table) for a few seconds until the rat settles. The fear of the height is found to reduce the tendency for the rat to climb down from the rat holder. Additional time (1-2 min) is given for the rat to settle, if needed.
   4. Insert the pedestal of the rat holder into the metal stand for its stable placement.
      NOTE: This setup allows changing the rat's viewing distance from the tablet screen.
   5. Turn on the camera to start video recording.
   6. Click on OKN Stripes Visualization Web Application from the desktop of the tablet screen and start running the program.
   7. Run the OKN stimuli in the left to right or right to left direction.
      NOTE: Rotation of the stripes in the clockwise direction activates the left eye, whereas rotation in the counter-clockwise direction activates the right eye. The initial direction of rotation was chosen randomly to avoid potential habituation.
   8. Observe the rat's head-tracking behavior.
   9. Start testing using the lowest spatial frequency (0.08 c/d), and then increase the spatial frequency in a step-wise (in the ascending) order.
   10. Notice the presence or absence of OKN responses.
   11. Continue testing, no resting period is given between different spatial frequencies.
   12. Stop video recording after completing all the spatial frequencies, and save the data using the rat identification number.
   13. Take the rat out of the holder and place it in the cage for approximately 30 min resting period between consecutive tests.
   14. Repeat the tests three times per rat.

2. Data analysis

1. Review the recorded video and determine each rat's visual acuity by finding the spatial frequencies at which the rat responded.
2. Record all responses (presence or absence of a clear head-tracking) in a spreadsheet.
3. Find the highest spatial frequency to which a rat responded from the three tests. This is considered the final visual acuity.
   NOTE: Since visual acuity measurement is subjective, data will be evaluated by two independent study personnel to confirm the visual acuity score for each rat¹². The results are cross-checked until the final score is agreed on. A positive OKN response is defined as the presence of a clear and sustained head-tracking activity. Random head-tracking (unrelated to the OKN visual stimuli) or the absence of any head movement is considered a negative OKN response.
4. Conduct statistical analysis to compare groups or between the two eyes (left eye vs right eye).
   NOTE: Review the video recordings to clarify that positive or negative responses is conducted as needed.

Results

The OKN testing was performed using retinal degenerate (RD) Royal College of Surgeons (RCS) rats and age-matched normal Long Evans (LE) rats. The LE rats (n = 4), were used for establishing the baseline data to determine visual acuity scores in normal rats using the new setup. Statistical analysis was carried out using Microsoft excel (mean ± standard deviation). LE rats exhibited robust head-tracking at spatial frequencies from 0.15 c/d to 0.33 c/d. To evaluate the reliability and effectiveness of the new OKN appar...

Discussion

OKN is a reflexive saw tooth motion of the eyes in response to a drifting stimulus, which is used as a tool to assess visual acuity in human subjects³. In animals, including primates and rodents, OKN testing is used as a quantitative measurement of visual function. The present study describes a novel, inexpensive OKN apparatus that can be easily set up in research laboratories for OKN behavioral testing in rats. OKN assessments in rodents were performed by different approaches. Previously, an inte...

Materials

Name	Company	Catalog Number	Comments
iPad Mini	Apple	A1489	Two iPad Mini's are used to display the OKN Stripes Visualization Software
Micro-camera/ Micro-Camera Attatchment	Lanon	B097H6WWDS	The micro-camera is used to record the experiment. The micro-camera attachment connects to the desk and holds the camera facing the rat. The head tracking responses are recorded and assessed at varying distances, spatial frequencies, and directions.
Plexiglass Tube/Rat Holder	Best acrylics	B07KMF31MC	The Plexiglass Tube is used to restrain the rat, with their head exposed, for the duration of the experiment. The tube is attached to another vertical plexiglass tube attachment to stabilize the rat holder during the experiment. The entire apparatus was designed and constructed in the lab.
Plexiglass Tube Attachment	Best acrylics	B07KMF31MC	This attachment holds the rat holder infront of the iPad screens, and allows the distance between the rat and iPad's to be manipulated.
Screen Holders	Kabcon	B08JLRPKQ1	Two screen holders are used to hold the iPad's up, in order to display the OKN Stripes Visualization Software to the rat.
OKN Stripes Visualization Web Application	The MIT License (MIT) Copyright (c) 2016 Anton Yakushin	https://antonyakushin.github.io/okn-stripes-visualization/	This application is a freely available softeware to display visual stimuli (black and white stripes) at different frequencies