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JoVE Journal

Immunology and Infection

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Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

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06:40 min

September 9th, 2014

September 9th, 2014

14,792 Views

0:05

Title

1:21

Purification of Fetal Liver Erythroblasts

2:32

Purification of TER119 Negative Erythroblasts

3:50

Transduced TER119 Negative Mouse Fetal Liver Erythroblast Culture

4:57

Results: Representative Flow Cytometric Analyses of Differentiated and Enucleated Transduced Erythroblasts

6:08

Conclusion

We present an in vitro mouse fetal liver erythroblast culture system that dissects the early and late stages of terminal erythropoiesis. This system facilitates functional analysis of specific genes in different developmental stages.

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