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JoVE Journal

Medicine

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Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

14.8K Views

08:07 min

June 23rd, 2015

June 23rd, 2015

14,790 Views

0:05

Title

1:11

Preparation for the Surgery

2:17

Surgery

3:43

The Microscope Set Up

4:30

Utilizing Rose Bengal Dye for Stroke Induction

6:37

Results: Vessel Occlusion and Stroke Confirmation

7:32

Conclusion

Here, we describe a semi-invasive optical microscopy approach for the induction of a Rose Bengal photothrombotic clot in the somatosensory cortex of a mouse in vivo. The technical aspects of the imaging procedure are described from induction of a photothrombotic event to application and data collection.

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