Results: Representative PTC and Sertoli Cell Culture
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The overall goal of this procedure is to isolate highly purified primary Sertoli cells and peritubular cells, or PTCs, from rat testes. This method can help answer key questions in the male reproductive immunology field such as how do Sertoli cell
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Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.