Palate Explant Culture and Confocal Time-lapse Imaging
3:28
Results: Representative Live Palate Explant Culture Imaging
5:16
Conclusion
Transcribir
The overall goal of this confocal live imaging method is to directly observe the cellular processes, the drive's secondary palate fusion during cranial facial development. Begin by separating the head from the body of the embryo under a dissecting
Inicia sesión o comienza tu prueba gratuita para acceder a este contenido
Here, we present a protocol for live imaging of mouse secondary palate fusion using confocal microscopy. This protocol can be used in combination with a variety of fluorescent reporter mouse lines, and with pathway inhibitors for mechanistic insight. This protocol can be adapted for live imaging in other developmental systems.