January 29th, 2018
•Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.
Videos relacionados
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
Three-dimensional Optical-resolution Photoacoustic Microscopy
Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants
High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
Isolation and Decellularization of a Whole Porcine Pancreas
High-resolution Patterned Biofilm Deposition Using pDawn-Ag43
High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados