Results: Study of the Lysosomal Functional Activity Using a pH Sensitive Dye
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Conclusion
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The overall goal of this procedure is to assess a relative loss of lysosomal acidity in the intestinal lysosomes of C.elegans. This method can help answer key questions in the field of autophagy by indirectly providing information about lysosomal
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A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA)