Immunofluorescence Staining of the Paraffin-embedded Epididymal Sections
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Isolation of Epididymosomes from the Mouse Caput Epididymis
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Immunofluorescence Staining of mECap18 Cells
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Isolation of Proteins from Conditioned Cell Culture Medium
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Results: Analysis of Epididymal Protein Synthesis and Secretion
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Conclusion
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This combined masters can answer key questions in the field of epididymal research, such as what protein targets are involved in the regulation of sperm maturation and storage. The main advantage of epididymal isolation technique is that it also a
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Here, we report the immunofluorescence localization of dynamin to illustrate the protocols for the detection of proteins in paraffin-embedded mouse epididymal sections and those of an immortalized epididymal cell line (mECap18). We also describe the protocols for the isolation of secretory proteins from both epididymal fluid and conditioned cell media.