October 25th, 2018
•Here, we present a protocol to prepare DNA samples from food and environmental microbiomes for concerted detection and subtyping of Salmonella through quasimetagenomic sequencing. The combined use of culture enrichment, immunomagnetic separation (IMS), and multiple displacement amplification (MDA) allows effective concentration of Salmonella genomic DNA from food and environmental samples.
Videos relacionados
Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
Chronic Salmonella Infected Mouse Model
High-Throughput Measurement and Classification of Organic P in Environmental Samples
One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bis(sulfosuccinimidyl) Suberate (BS3)
Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Precise, High-throughput Analysis of Bacterial Growth
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados