Results: Immunoblotting Ratios Strongly and Positively Correlated with Intensity Readings from Quantitative IF
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Conclusion
Transcripción
This method can help answer key questions in the field of cancer pathology, by quantifying biomarker proteins from routinely processed biopsy material. The main advantages of this technique in relation to conventional immunohistochemistry, are tha
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We describe the use of quantitative immunoblotting to validate immunofluorescence histology coupled with image analysis as a means of quantifying a protein of interest in formalin-fixed, paraffin-embedded (FFPE) tissue samples. Our results demonstrate the utility of immunofluorescence histology for ascertaining the relative quantity of biomarker proteins in routine biopsy samples.