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Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples

8.7K Views

09:58 min

January 7th, 2019

DOI :

10.3791/58735-v

January 7th, 2019


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Quantitative Immunoblotting

Chapters in this video

0:04

Title

1:29

Building a Cell-Line Tissue Microarray (TMA)

3:37

Sample Staining by Immunofluorescence

4:27

Quantitative Immunoblotting of Cell Lines

7:48

Results: Immunoblotting Ratios Strongly and Positively Correlated with Intensity Readings from Quantitative IF

9:22

Conclusion

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