Mouse Allergic Lung Inflammation Induction and Human Peripheral Blood CD4+ T Cell Labeling
2:42
CD4+ T Cell Adoptive Transfer and Lung Tissue Preparation
5:26
Murine Lung Light-sheet Fluorescence Microscopy
6:43
Post-image Processing and Lung-accumulated Human Cell Quantification
8:12
Results: Representative Human CD4+ T Cell Lung Accumulation Analysis
9:50
Conclusion
Transcribir
Our technique allows the precise imaging of lung-accumulated human immune cells in in vivo inflammatory conditions providing exciting insights into the process of T cell lung homing. This protocol supports translational research on T cell lung hom
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The here introduced protocol allows characterization of the lung homing capacity of primary human lymphocytes under in vivo inflammatory conditions. Pulmonary infiltration of adoptively transferred human immune cells in a mouse model of allergic inflammation can be imaged and quantified by light-sheet fluorescence microscopy of chemically cleared lung tissue.