Casting Upright Fibrin Gel Droplets for the Characterization of Native FT and Ex Vivo Culture
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Native FT and Ex Vivo Culture End-Point
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Whole-Mount Immunofluorescence Staining
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Results: PDR Ex Vivo Culture Induced Sprouting of CD31-positive Endothelium and Vasculature Preservation in Response to VEGFA
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Conclusion
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Diabetic retinopathy is the most common microvascular complication of diabetes.This method can help answer key questions on the pathophysiology of the end stage disease proliferative diabetic retinopathy.This technique allows the deconstruction of
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Here, we present a protocol to study the pathophysiology of proliferative diabetic retinopathy by using patient-derived, surgically-excised, fibrovascular tissues for three-dimensional native tissue characterization and ex vivo culture. This ex vivo culture model is also amenable for testing or developing new treatments.