Results: Replating of Differentiated iPSC-Derived Neurons
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Conclusion
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This protocol facilitates the use of human pluripotent stem cell-derived neurons for high content screening applications. It is particularly well suited for assaying synapses, which in human neurons require lengthy culture periods. We demonstrate
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This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. The procedure facilitates imaging-based assays of neurites, synapses, and late-expressing neuronal markers in a format compatible with light microscopy and high-content screening.