Results: Successful Knockout of GM-CSF in CAR-T Cells
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Conclusion
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Our protocol describes the genetic modification of CAR-T cell during their manufacturing via the CRISPR technology to generate more effective and less toxic product. An advantage of this technique is that CAR-T cell production and genetic manipula
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Here, we present a protocol to genetically edit CAR-T cells via a CRISPR/Cas9 system.