April 24th, 2020
•Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.
Tags
Videos relacionados
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados