The pial surface is a unique progenitor zone in the CNS that is receiving increasing attention. Herein, we detail a method for rapid genetic manipulation of this progenitor zone using a modified electroporation method. This procedure can be used for cellular and molecular investigations of cell lineages and signaling pathways involved in cell differentiation and to elucidate the fate and properties of daughter cells.
Videos relacionados
In vivo Electroporation of Developing Mouse Retina
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
Neonatal Subventricular Zone Electroporation
Subtype-selective Electroporation of Cortical Interneurons
Spinal Cord Neurons Isolation and Culture from Neonatal Mice
In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice
Cochlear Surface Preparation in the Adult Mouse
Continuous Video Electroencephalogram during Hypoxia-Ischemia in Neonatal Mice
Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
Author Spotlight: High-Resolution Imaging of Mouse Neonate Brains – A Micro-CT Protocol with Lugol's Solution Contrast Agent
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados