Results: In Situ Ultrastructure Exploration of Murine Megakaryopoiesis
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Conclusion
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The overall goal of this procedure is to observe the ultra structure of megakaryocytes located within the mouse bone marrow, and to quantify the different maturation stages using high-resolution transmission electron microscopy. The main advantage
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Here, we present a protocol to analyze ultrastructure of the megakaryocytes in situ using transmission electron microscopy (TEM). Murine bone marrows are collected, fixed, embedded in epoxy resin and cut in ultrathin sections. After contrast staining, the bone marrow is observed under a TEM microscope at 120 kV.