Tissue Harvesting and 2D/3D Immunofluorescence Staining
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Results: An Ex Situ Skin Scarring Assay to Visualize Scar Development
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Conclusion
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Previously used in vitro or ex vivo models lack dermal cell components and complexity of skin tissue. This ex situ SCAD assay overcomes a set limitation and enables studying the development of scars outside of wounded animal. The SCAD assay allows
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This protocol describes the generation of a skin-fascia explant termed "SCar like tissue in A Dish" or SCAD. This model allows unprecedented visualization of single fibroblasts during scar formation.