October 4th, 2013
•Two complementary methods based on flow cytometry and microscopy are presented which enable the quantification, at the single cell level, of the dynamics of gene expression induced by the activation of a MAPK pathway in yeast.
Vidéos Connexes
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1 Video (Video) | JoVE
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2 Video (Video) | JoVE
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3 Video (Video) | JoVE
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency Video (Video) | JoVE
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris Video (Video) | JoVE
Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast Video (Video) | JoVE
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells Video (Video) | JoVE
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia Video (Video) | JoVE
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System Video (Video) | JoVE
Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells Video (Video) | JoVE