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JoVE Journal

Immunology and Infection

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In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers

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13:10 min

August 1st, 2014

August 1st, 2014

11,555 Views

0:05

Title

1:49

In Situ Staining of Cerebral Sections with MHC Class II (IAs)/PLP 139-151 Dextramers

6:28

Analysis of Dextramer+ (dext+) CD4+ T Cells by Laser Scanning Confocal Microscopy (LSCM)

10:50

Results: In Situ Detection of Antigen-specific Autoreactive CD4 T Cells by Direct Staining with MHC Class II Dextramers

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Conclusion

The protocol to detect self-reactive CD4 T cells in brain and heart by direct staining with major histocompatibility complex class II dextramers has been described in this report. For comprehensive analysis, a reliable method to enumerate the frequencies of antigen-specific CD4+ T cells in situ is also devised.

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