Results: Images of Cells in the Intermediate Steps of the Differentiation Protocol
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Conclusion
Transcription
The overall goal of this protocol is to provide neurobiologists and neurovirologists with an easy, reproducible method to generate differentiated human neurons for the use in subsequent in vitro assays. This method can help to answer key questions
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It is critical in neurobiology and neurovirology to have a reliable, replicable in vitro system that serves as a translational model for what occurs in vivo in human neurons. This protocol describes how to culture and differentiate SH-SY5Y human neuroblastoma cells into viable neurons for use in in vitro applications.