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Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

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10:12 min

November 13th, 2015

November 13th, 2015

12,653 Views

0:05

Title

0:45

Isolation of Skeletal Muscle

3:47

Isolation of Mitochondria from Skeletal Muscle

7:50

Mitochondria Membrane Integrity Assay

8:42

Results: Membrane Integrity Measurements of the Isolated Preparation

9:31

Conclusion

Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.

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