The overall goal of this experimental procedure is to observe the cyclic AMP dependent regulation of fibroglast migration by MRP4.High contact microscopy can help answer key questions in the field of cell biology.For example, in addition to confer
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MRP4 regulates various cyclic nucleotide-dependent signaling events including a recently elucidated role in cell migration. We describe a direct, but multifaceted approach to unravel the downstream molecular targets of MRP4 resulting in identification of a unique MRP4 interactome that plays key roles in the fine-tuned regulation of fibroblast migration.