Preparation of Samples and Environmental Chamber Set-up
2:01
Microscope and Imaging Set-up
4:03
Results: Time Lapse Tracking of Single Cells to Study Anti-cancer Therapeutics
5:35
Conclusion
Transcription
The overall goal of this procedure is to observe anti-cancer therapeutic response in single cells in culture. This method can answer key questions in the cancer research field, including heterogeneous therapeutic response and cell fate. To begin,
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Here, we describe a method of long-term time-lapse microscopy to longitudinally track single cells in response to anti-cancer therapeutics.