CHIK VLP Expression Using Baculovirus/Insect Cell System
3:18
Sedimentation and Purification of VLPs and SVPs
4:42
Results: Successful Expression and Purification of VLPs
5:23
Conclusion
Transcription
The overall goal of this procedure is to purify virus-like particles, or VLPs, expressed and secreted by mammalian or insect-cell expression systems. This method can answer key questions in a vaccinoly's field, such as how to express and purify co
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Here, we present a protocol for synthesizing virus-like particles using either baculovirus or mammalian expression systems, and ultracentrifugation purification. This highly customizable approach is used to identify viral antigens as vaccine targets in a safe and flexible manner.