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Expression and Purification of Virus-like Particles for Vaccination

DOI :

10.3791/54041-v

June 2nd, 2016

June 2nd, 2016

20,677 Views

1Center for Vaccines and Immunology, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia

Here, we present a protocol for synthesizing virus-like particles using either baculovirus or mammalian expression systems, and ultracentrifugation purification. This highly customizable approach is used to identify viral antigens as vaccine targets in a safe and flexible manner.

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