Selection and Relaxation of Nutritionally Conditioned Animals
2:12
Amputation
3:44
Assessing Regeneration with the Nematostella Regeneration Staging System (NRSS)
5:47
Results: Effects of Chloroquine, and Inhibitor of Autophagy
6:35
Conclusion
Transcription
The overall goal of this procedure is to illustrate the predictable series of morphological changes that occurred during the regeneration of a complete polyp from a tiny section of tissue removed from the aboral tip of nematostella vectensis This
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Here we demonstrate how to induce and monitor regeneration in the Starlet Sea Anemone Nematostella vectensis, a model cnidarian anthozoan. We demonstrate how to amputate and categorize regeneration using a morphological staging system, and we use this system to reveal a requirement for autophagy in regenerating polyp structures.