Protein Purification via Immobilized Cobalt Affinity Resin (ICAR)
5:04
Nondenaturing Polyacrylamide Gel Electrophoresis (PAGE)
8:09
Results: A Combined Approach for Assaying Proteasome Assembly
9:24
Conclusion
Transcription
The overall goal of this experiment is to analyze assembly of a multi-protein complex such as the proteasome, using recombinant subunits, and nondenaturing Polyacrylamide Gel Electrophoresis, or PAGE.This method can help answer key questions in th
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This protocol uses both subunit coexpression and postlysis subunit mixing for a more thorough examination of recombinant proteasome assembly.