Functionalization of Coverslip and Cantilever Surface
4:31
Stepwise Polyprotein Preparation with Controlled Sequences
5:44
AFM Experiment Measurement and Data Analysis
6:54
Results: AFM-based SMFS Measurements, UV-Vis Absorbance Spectra and SDS-PAGE Gel Results
7:51
Conclusion
Transcription
Our enzymatic method use protein oligomer with a controlled number of polymerization degree. It is a perfect sample preparation for single-molecule force spectroscopy study as well as protein-based material construction. Our method does not introd
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Here, we present a protocol to conjugate protein monomer by enzymes forming protein polymer with a controlled sequence and immobilize it on the surface for single-molecule force spectroscopy studies.