Dissection of Epicardial Layer and Isolation of Epicardial Derived Cells
4:10
Culture of EPDCs
5:32
Induction of Epithelial to Mesenchymal Transition in EPDCs
6:31
Results: Isolation of EPDCs from Human Adult Cardiac Tissue and Induction and Validation of EMT in EPDCs
8:17
Conclusion
Transcription
The overall goal of this procedure is to isolate and culture human epicardial-derived cells in order to study epicardial cell behavior. This method can help answer key questions in the field of cardiac research, such as how is the epicardium activ
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The epicardium plays a crucial role in the development and repair of the heart by providing cells and growth factors to the myocardial wall. Here, we describe a method to culture human primary epicardial cells that enables the study and comparison of their developmental and adult characteristics.