August 2nd, 2018
•Here we provide a detailed protocol to carry out in vivo cardiac gene editing in mice using recombinant Adeno-Associated Virus(rAAV)-mediated delivery of CRISPR. This protocol offers a promising therapeutic strategy to treat dystrophic cardiomyopathy in Duchenne muscular dystrophy and can be used to generate cardiac-specific knockout in postnatal mice.
Vidéos Connexes
Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery Video (Video) | JoVE
High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors Video (Video) | JoVE
Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice Video (Video) | JoVE
Production and Detection of Reactive Oxygen Species ROS in Cancers Video (Video) | JoVE
Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice Video (Video) | JoVE
An Orthotopic Bladder Cancer Model for Gene Delivery Studies Video (Video) | JoVE
Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis Video (Video) | JoVE
Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease Video (Video) | JoVE
Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector Video (Video) | JoVE
Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration Video (Video) | JoVE