Results: Two-Step Purification Results in Effective Isolation of F1-ATPase
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Conclusion
Transcription
The purification of F1-ATPase is based on a classical biochemical methods which proved to be crucial for our understanding of the mechanism of ATP production by other ATP synthases. The main advantage of this technique is that it produces a highly
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This protocol describes the purification of F1-ATPase from the cultured insect stage of Trypanosoma brucei. The procedure yields a highly pure, homogeneous, and active complex suitable for structural and enzymatic studies.