September 14th, 2014
•We present a protocol of how to collect and process electron cryo-tomograms of whole mitochondria. The technique provides detailed insights into the structure, function, and organization of large membrane protein complexes in native biological membranes.
Tags
Vidéos Connexes
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo
Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells
Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging