Fabrication of Single-well Polydimethylsiloxane Plates and Square Compartments
2:52
Cell Culture
4:28
Imaging
7:14
Results: Fluctuations Data of the Nucleus in Response to Chemical and Strain Cues
8:31
Conclusion
Transcription
We have previously designed a device to apply mechanical strain to adherent cells. In the current work, we have redesigned the geometry of the substratum and customized the setup for high-resolution imaging of these strain cells. The main advantag
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Using a previously designed device to apply mechanical strain to adherent cells, this paper describes a redesigned substratum geometry and a customized apparatus for high-resolution single-cell imaging of strained cells with a 100x oil immersion objective.