Results: 3D Organoids from Patient-derived Xenograft and Primary Tumor Tissue
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We describe strategies and step-by-step protocols to establish serial 3D patient-derived organoids of bone metastatic prostate cancer. Our optimized protocols are practical to set up 3D cultures for experiments using limited starting material dire
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Three-dimensional cultures of patient BMPC specimens and xenografts of bone metastatic prostate cancer maintain the functional heterogeneity of their original tumors resulting in cysts, spheroids and complex, tumor-like organoids. This manuscript provides an optimization strategy and protocol for 3D culture of heterogeneous patient derived samples and their analysis using IFC.