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Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

11.2K Views

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05:37 min

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December 28th, 2019

December 28th, 2019

•
11,220 Views

0:04

Title

0:32

Egg Laying

1:13

Starvation Treatment

2:10

Oenocyte Dissection

3:10

Lipid Droplet Staining

4:02

Mounting and Imaging

4:41

Results: Representative Lipid Droplet Staining

5:06

Conclusion

Transcription

This method of an oenocyte dissection and lipid droplet staining can be used to realize the appearance of lipid droplet in drosophila larvae oenocyte under normal and stressed conditions. This method provide a useful tool for studying lipid metabo

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Presented here are detailed methods for the dissection and lipid droplet staining of oenocytes in Drosophila larvae using BODIPY 493/503, a lipid droplet-specific fluorescent dye.

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