Functionalization of Coverslip and Cantilever Surface
4:31
Stepwise Polyprotein Preparation with Controlled Sequences
5:44
AFM Experiment Measurement and Data Analysis
6:54
Results: AFM-based SMFS Measurements, UV-Vis Absorbance Spectra and SDS-PAGE Gel Results
7:51
Conclusion
文字起こし
Our enzymatic method use protein oligomer with a controlled number of polymerization degree. It is a perfect sample preparation for single-molecule force spectroscopy study as well as protein-based material construction. Our method does not introd
このコンテンツにアクセスするにはサインインするか無料トライアルを開始してください
Here, we present a protocol to conjugate protein monomer by enzymes forming protein polymer with a controlled sequence and immobilize it on the surface for single-molecule force spectroscopy studies.