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OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

6.6K Views

08:34 min

February 5th, 2020

DOI :

10.3791/60774-v

February 5th, 2020


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OaAEP1

Chapters in this video

0:05

Introduction

0:43

Functionalization of Coverslip and Cantilever Surface

4:31

Stepwise Polyprotein Preparation with Controlled Sequences

5:44

AFM Experiment Measurement and Data Analysis

6:54

Results: AFM-based SMFS Measurements, UV-Vis Absorbance Spectra and SDS-PAGE Gel Results

7:51

Conclusion

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