We'll describe a stencil-based lithography-free single-cell micropattern method that overcomes many obstacles associated with photolithography and soft lithography. The stencils are reusable and the process is inexpensive and customizable. This me
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This protocol introduces a lithography-free micropatterning method that is simple and accessible to those with a limited bioengineering background. This method utilizes customized laser-cut stencils to micropattern extracellular matrix proteins in a shape of interest for modulating cell morphologies. The procedure for micropatterning is demonstrated using induced pluripotent stem cell derived cardiomyocytes.