Isolation of Nuclei from Remaining Cytoplasmic Contaminants
3:05
Nuclei Lysis and Histone Extraction
4:39
Results: Qualitative & Quantitative Analysis of the Identified Histones
6:36
Conclusion
Transcription
Our protocol uncovers epigenetic markers for sorghum plant development and drought resistance. Such molecular understanding will help develop better solutions to adapt crops for extreme climates in the future. This protocol generates high purity h
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The protocol has been developed to effectively extract intact histones from sorghum leaf materials for profiling of histone post-translational modifications that can serve as potential epigenetic markers to aid engineering drought resistant crops.