Differentiation of Murine 3T3-L1 Fibroblasts into Adipocytes and Preparation of Precoated Plates
3:18
Preparation of the Transfection Mix and 3T3-L1 Adipocytes
6:28
Results: Differentiation, Protein Silencing, Micro-RNA Overexpression and Effects of Protein or Micro-RNA Modulation in 3T3-L1 Adipocytes
8:35
Conclusion
Transcription
This protocol makes it possible to manipulate proteins, or microRNA expression, in differentiated adipocytes to study their role in adipocytes function. This reverse-transfection method is a simple, economical, and highly efficient method to trans
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Presented here is a protocol to deliver oligonucleotides such as small-interfering RNA (siRNA), micro-RNA mimics (miRs), or anti-micro-RNA (anti-miR) into mature adipocytes to modulate protein and micro-RNA expression.