Endogenous Streptavidin, Biotin Blocking, Primary Antibody Staining, and LR-ExM Specific Secondary Antibody Staining
2:29
Additional Anchoring, Gelation, and Digestion
4:26
Post-Digestion Fluorescence Staining, Expansion, and Imaging
5:34
Results: Labelling the Target Proteins Using Trifunctional Anchors in Label Retention Expansion Microscopy
7:01
Conclusion
Transcription
One major limitation of expansion microscopy is fluorescence slows after polymerization and digestion. Label retention expansion microscopy, we call it LR-ExM, uses tri functional anchors, which are in order to polymerization and digestion. After
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A protocol of label retention expansion microscopy (LR-ExM) is demonstrated. LR-ExM uses a novel set of trifunctional anchors, which provides better labeling efficiency compared to previously introduced expansion microscopies.