Design and Synthesis of DNA Library and Primers and Infectious and Non‐Infectious Virus Samples
1:46
In Vitro Selection or SELEX Process: Initial Round
6:38
Subsequent Selection Rounds
7:55
Monitoring the SELEX Process
8:46
High‐Throughput Sequencing, Sequencing Analysis, and Aptamer Binding Validation and Assays
10:38
Results: SELEX Process of Aptamers for Distinguishing Infectious from Non‐Infectious Viruses
11:42
Conclusion
Transcription
This protocol describes how to select DNA aptamers that can distinguish infectious viruses versus non-infectious viruses in order to obtain sensing molecule able to inform the infectability of clinical or environmental samples. This technique can
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We provide a protocol that can be generally applied to select aptamers that bind to infectious viruses only and not to viruses that have been rendered non-infectious by a disinfection method or to any other similar viruses. This opens the possibility of determining infectivity status in portable and rapid tests.