Design and Synthesis of DNA Library and Primers and Infectious and Non‐Infectious Virus Samples
1:46
In Vitro Selection or SELEX Process: Initial Round
6:38
Subsequent Selection Rounds
7:55
Monitoring the SELEX Process
8:46
High‐Throughput Sequencing, Sequencing Analysis, and Aptamer Binding Validation and Assays
10:38
Results: SELEX Process of Aptamers for Distinguishing Infectious from Non‐Infectious Viruses
11:42
Conclusion
Transkript
This protocol describes how to select DNA aptamers that can distinguish infectious viruses versus non-infectious viruses in order to obtain sensing molecule able to inform the infectability of clinical or environmental samples. This technique can
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
We provide a protocol that can be generally applied to select aptamers that bind to infectious viruses only and not to viruses that have been rendered non-infectious by a disinfection method or to any other similar viruses. This opens the possibility of determining infectivity status in portable and rapid tests.