S'identifier

JoVE Journal

Genetics

This content is Free Access.

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

4.6K Views

06:04 min

September 16th, 2022

September 16th, 2022

4,618 Views

0:05

Introduction

0:43

Gonad Dissection

4:23

Results: Visualizing Meiotic Chromosomes in C. elegans

5:10

Conclusion

Transcription

This is a commonly used method for C. elegans gonad dissection followed by freeze crack, which produces germline samples for immunofluorescence via antibody staining, or for simple DAPI staining to visualize DNA. This protocol has been successful for undergraduates in a research lab and in a course-based undergraduate research experience.

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