Bioreactor Fabrication: Casting of the PDMS Rack from the Aluminum Negative Masters
2:14
Bioreactor Fabrication: PDMS Rack Removal from the Aluminum Negative Master Casts
3:28
Bioreactor Fabrication: Stable Post Tracker (SPoT) Fabrication
5:39
Human Engineered Cardiac Tissue (hECT) Fabrication
7:52
Baseplate Removal
8:44
Human Engineered Cardiac Tissue (hECT) Functional Characterization
10:27
Results: Data Acquisition from the Bioreactor
11:53
Conclusion
Transcription
Our invitro model of three-dimensional stem cell derived human heart muscle improves the biofidelity of traditional two-dimensional cell culture while eliminating the interspecies differences associated with laboratory animal testing. Our multi-ti
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Three-dimensional cardiac tissues bioengineered using stem-cell-derived cardiomyocytes have emerged as promising models for studying healthy and diseased human myocardium in vitro while recapitulating key aspects of the native cardiac niche. This manuscript describes a protocol for fabricating and analyzing high-content engineered cardiac tissues generated from human induced pluripotent stem-cell-derived cardiomyocytes.